Bewo-CAS9 cell line

×

Subscribe Us

By subscription, you consent to allow Ubigene Biosciences to store and process the information provided above to deliver the latest news, research spotlight, and promotions. You can unsubscribe from these communications at anytime.
location: Home > Products > Cas9 Stable Cell Lines > Bewo-CAS9
All products
  • All products
  • Red Cotton™ gRNA Plasmid Bank
  • KO Cell Lines
  • Wild-type Cell Lines
  • Cas9 Stable Cell Lines
  • Luciferase Stable Cell Lines
  • EGFP Stable Cell Lines
  • Stem Cell Lines
  • Transfection Culture Medium
  • Cell Monoclonal Culture Medium
  • EZ-Stem™ Cell Culture Medium
  • CRISPR-iScreen™ Library Plasmid
  • CRISPR-iScreen™ Library Virus
  • CRISPR-iScreen™ Cell Pool
  • EZ-cryo™ cell freezing medium
  • Kits
  • Lentivirus

Bewo-CAS9 Order

Bewo-CAS9 cell line
Catalog#
YC-D018-Cas9-H
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Datasheet
Bewo-CAS9 cell line
Order

Detailed Product Information

Product category
Cas9 Stable Cell Lines
cell name
Bewo-CAS9
Cell Morphology
Epithelial-like, adherent
Passage ratio
1:3~1:4
Species
Human
Expression gene
Cas9
Resistance gene
hygro
Construction method
Lentivirus
Mycoplasma test
negative
Culture method
50%DMEM/F12+40%FBS+10%DMSO
Description
Use Cas9 gene expressing lentivirus constructed by Ubigene to infect the Bewo cell line, screen the cells based on the resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a Bewo-CAS9 cell line that can maintain long-term, stable and high expression.
Product Validation Data(RT-QPCR)
Sample Name Target Name Cт Mean ΔCт
BeWo-CAS9 Cas9 17.07200432 4.40266323
BeWo-CAS9 β-actin 12.66934109
Bewo Cas9 35.19368744 22.51105595
Bewo β-actin 12.68263149
Cutting Efficiency Validation
Bewo-CAS9 cell line Product Validation Data(RT-QPCR)
Note: The above figure shows the sequencing peaks of the BeWo-CAS9 stable cell pool which is electroporated by TRPV6 gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.
Loci STR Info (Sample Cell)
Sample Cell Line:Bewo
STR Info (Cell bank)
Cell Line:BEWO
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 10111011
D13S317 911911
D7S820 10121012
D16S539 13141314
VWA 16161616
TH01 99.399.3
AMEL XYXY
TPOX 8888
CSF1PO 11121112
D12S391 1724
FGA 222324.0
D2S1338 2021
D21S11 3030
D18S51 1416
D8S1179 1212
D3S1358 1515
D6S1043 1111
PENTAE 812
D19S433 1315
PENTAD 912
D1S1656 1416
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Product Advantage

  • Easy to use:Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
    01
  • High-efficient KO:These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
    02
  • High-quality cell:Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
    03
Bewo-CAS9 cell line
Bewo-CAS9
Catalog#
YC-D018-Cas9-H
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

KO Cell Lines

  • ERVFRD-1 Knockout cell line(BeWo)

    ERVFRD-1 Knockout cell line(BeWo)

    Organism: Human
    Gene Name: ERVFRD-1
    Gene ID: 405754
    Size: 1*10^6
    Catalog#: YKO-H208
    YKO-H208(BeWo)
  • TRPV6 Knockout cell line (BeWo)

    TRPV6 Knockout cell line (BeWo)

    Organism: Human
    Gene Name: TRPV6
    Gene ID: 55503
    Size: 1*10^6
    Catalog#: YKO-H124
    YKO-H124(BeWo)

Luciferase Stable Cell Lines

Wild-type Cell Lines

Related Popular Products

KO Cell Lines

  • ERVFRD-1 Knockout cell line(BeWo)

    ERVFRD-1 Knockout cell line(BeWo)

    Organism: Human
    Gene Name: ERVFRD-1
    Gene ID: 405754
    Size: 1*10^6
    Catalog#: YKO-H208
  • TRPV6 Knockout cell line (BeWo)

    TRPV6 Knockout cell line (BeWo)

    Organism: Human
    Gene Name: TRPV6
    Gene ID: 55503
    Size: 1*10^6
    Catalog#: YKO-H124

Luciferase Stable Cell Lines

Wild-type Cell Lines


Contact us